Of course also some of us here in GDSE are able to present things which are normally invisible, but they make art. They very likely can be found among astronomy and astrophotography hobbyists. Unfortunately I do not know does it solve your current problem.įor more help contact people who have worked with the same problem. To merge several images, go to Image > Color > Merge Channels. To get what you expect you need other software than Photoshop as said by joojaa and Billy Kerr - the latter even picked one freebie (=DeepSkyStacker) which at least knows how to use RAWs. Getting Started To pseudocolor the image, go to Image > LookUp Table and select desired color. The only way to extract something which is now invisible is to start from original RAW images which have got no processing. Revealing what there originally was collected is impossible if images are processed to good looking 8 bit/channel usual photos - a vast amount of original info is thrown overboard without asking because it was probably considered to be only noise - too weak to be interesting - and non-linear dynamics compression has mixed the rest together in a non-reversible way. The goal of this is to visualize the overlay of the nuclei in the BF image with the three channel fluorescence data from the fluorescent labels. Merge multi-channel images ZEN exported version 1.2.0 2018.05. Only summing the original unmangled data can be considered as something you expect. Contribute to hzqfox/ImageJmacros development by creating an account on GitHub. We do not have a slightest idea what camera system you had when you took the photos and what mangling the photos have got between the exposure ending and being "ready to be combined in Photoshop". The comments revealed that you want to combine 2 grayscale photos to get one which is a substitute for a photo which was taken with longer, say double length exposure. User joojaa guessed it and wrote a warning. Description: Repairs displacement of fluorescence channels in bacterial images. Unfortunately the question didn't present what you want. : Version 16 (works with ImageJ 1.50d) : Version 15. Impressum Dustin Schweitzberger Dipl.-Ing. You have got a perfect answer from user Alexander Samual Dunnett for the presented question.
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